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Abstract

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Short Paper | Special issue | Vol 97, No. 1, 2018, pp. 612 - 620
Published online: 2nd April, 2018
DOI: 10.3987/COM-18-S(T)48
Synthesis of Alkynyl C-Nucleotide Triphosphates Toward Enzymatic Elongation of Artificial DNA

Yutaro Oda, Junya Chiba,* and Masahiko Inouye

*Graduate School of Medicines and Pharmaceutical Sciences for Research, Toyama University, 2630 Sugitani, Toyama, Toyama 930-0194, Japan

Abstract

Four types of alkynyl C-nucleotide triphosphates were synthesized, in which heterocyclic compounds as a non-natural nucleobase were tethered to deoxyribose through acetylene bonds. The non-natural nucleobases are D*, T*, G*, and C*, corresponding to natural A, T, G, and C, respectively, from a hydrogen-bonding pattern of view. All the non-natural triphosphates were found to be a good substrate for terminal deoxynucleotidyl transferase (TdT), a natural nucleic acid-relevant enzyme.

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