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Paper | Regular issue | Vol 38, No. 8, 1994, pp.1757-1774
Published online, 1st January, 1970
DOI: 10.3987/COM-93-S161
Nuclear Magnetic Resonance Studies of Hyaluronan (HA): Evidence of Competitive Inhibition of Interchain Associations by Phospholipids which may result in decreased Anti-Inflammatory and Cartilage Protecting Properties of HA

Peter Ghosh,* Nongporn Hutadilok, and Aldo Lentini

*Raymond Purves Bone & Joint Research Laboratories, The Royal North Shore Hospital of Sydney, University of Sydney, St. Leonards, NSW 2065, Australia


The chain flexibility of solutions of hyaluronan (HA) of different molecular weights was determined by 1H-nmr spectroscopy in the absence and presence of the phospholipid Dipalmitoyl DL-α-phosphatidylchline (DPC). Sonication of high or low molecular weight HA with DPC for periods up to 60 min markedly increased HA chain flexibility as determined by observing the spin-spin relaxation times for the glucosamine acetyl group protons. From these data it is proposed that DPC competes for those hydrophobic centres along the HA chain which are normally responsible for the inter and intra chain interactions and which confer stiffiness to the HA molecule.