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Note | Regular issue | Vol 81, No. 4, 2010, pp.985-990
Published online, 9th February, 2010
DOI: 10.3987/COM-09-11895
Peptide Mass Spectrometry by Ionic Probe Attachment Ionization Using NHS-TMpybox and Mal-TMpybox

Fumihiro Ito and Kentaro Yamaguchi*

*Faculty of Pharmaceutical Sciences at Kagawa Campus, Tokushima Bunri University, 1314-1 Shido, Sanuki City, Kagawa 769-2193, Japan


The application of a new ionization method that uses metal-complex-based ionization probes containing the 2,6-bis(oxazolinyl)pyridine (pybox) ligand for bioactive peptides is presented. Bradykinin, angiotensin-I, angiotensin-II, and nociceptine as well as a couple of pentapeptides were analyzed. This method was proven to effectively ionize large complex molecules, including biomolecules.