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Paper | Special issue | Vol 90, No. 1, 2015, pp.432-441
Published online, 23rd July, 2014
DOI: 10.3987/COM-14-S(K)41
Base Pair Recognition Ability of 2-(Methylamino)pyrimidin-4-yl Nucleobase in Parallel Triplex DNA

Yoshiyuki Hari,* Satoshi Kashima, Yuya Matsuda, Akihiro Sakata, Ryutaro Takamine, Shin Ijitsu, and Satoshi Obika*

*Graduate School of Pharmaceutical Science, Osaka University, 1-6 Yamadaoka, Suita, Osaka 560-0871, Japan

Abstract

A phosphoramidite bearing a 2-(methylamino)pyrimidin-4-yl nucleobase was synthesized and the modified oligonucleotide (triplex-forming oligonucleotide, TFO) was successfully obtained using the phosphoramidite on an automated DNA synthesizer. UV-melting experiment of triplex DNA between the synthesized TFO and duplex DNA targets indicated that the 2-aminopyrimidin-4-yl unit in TFO could be a useful core structure for recognition of a TA base pair in duplex DNA.