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Short Paper | Special issue | Vol 97, No. 2, 2018, pp.1219-1225
Published online, 29th March, 2018
DOI: 10.3987/COM-18-S(T)73
Isolation of Aaptic Acid from the Marine Sponge Aaptos lobata and Inhibitory Effect of Aaptamines on RANKL-Induced Formation of Multinuclear Osteoclasts

Arina Fukumoto, Yuki Hitora, Aika Kai, Hikaru Kato, Esther D. Angkouw, Remy E. P. Mangindaan, Nicole J. de Voogd, and Sachiko Tsukamoto*

*Graduate School of Pharmaceutical Sciences, Kumamoto University, 5-1 Oe-hon-machi, Kumamoto 862-0973, Japan

Abstract

A new aaptamine congener, aaptic acid (1), was isolated from the marine sponge Aaptos lobata collected in Indonesia, together with aaptamine (2), demethyl(oxy)aaptamine (3), and isoaaptamine (4). The chemical structure of 1 was assigned on the basis of NMR data. The isolated compounds were tested for inhibitory activities against osteoclastogenesis in RAW264 cells. At 5 μM concentration, 2-4 inhibited RANKL-induced multinuclear osteoclast formation by 25%, 54%, and 17%, respectively. In contrast, 1 did not inhibit osteoclastogenesis even at 50 μM.