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Paper | Regular issue | Vol 100, No. 4, 2020, pp.568-584
Published online, 24th March, 2020
DOI: 10.3987/COM-20-14228
Identification and Quantification of Alkaloid Compounds from Different Parts and Production Areas of Datura metel L.

Yang Shi Hui, Liu Yan, Wang Qi, Sun Yan Ping, Guan Wei, Liu Yuan, Yang Bing You,* and Kuang Hai Xue*

*Key Laboratory of Chinese Materia, Heilongjiang University of Chinese Medicine, Ministry of Education, Harbin 150040, China

Abstract

An ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS/MS) method was used to identify alkaloids in six parts of Datura metel L. (D. metel L.). 65 Alkaloids were tentatively identified. Meanwhile, a rapid, sensitive and reliable ultra-high-performance liquid chromatography tandem triple quadrupole mass spectrometry (UPLC-Q-TRAP-MS/MS) technique had developed to determine the contents of 22 alkaloids in five minutes, with good precision and repeatability. This method provided a feasible solution for the content determination of alkaloid in D. metel L.